Chromosomal Assignment of Short cDNA Sequences by PCR Using Overlapping and Tailed Short Primers | Zendy

KeonSang Chae | Zendy; Katsuji Murakawa | Zendy; Kousaku Okubo | Zendy; Kenichi Matsubara | Zendy

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Chromosomal Assignment of Short cDNA Sequences by PCR Using Overlapping and Tailed Short Primers

Author(s) -

KeonSang Chae,

Katsuji Murakawa,

Kousaku Okubo,

Kenichi Matsubara

Publication year - 1994

Publication title -

dna research

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.647

H-Index - 98

eISSN - 1756-1663

pISSN - 1340-2838

DOI - 10.1093/dnares/1.3.149

Subject(s) - biology , complementary dna , genetics , genbank , microbiology and biotechnology , in silico pcr , gene , polymerase chain reaction , multiplex polymerase chain reaction

Overlapping primers and tailed short primers are effective agents for mapping very short cDNA sequences. By using such primers, human cDNAs as short as 32 nucleotides in length can produce PCR bands. Using these and other primers of ordinary size, 44 cDNAs were assigned to chromosomes, of which 24 were assigned to single chromosomes, and 2 were assigned to two chromosomes and two were assigned to three chromosomes, respectively. Among the 24 cDNAs, all of which matched GenBank entries, 6 cDNAs were observed to map to the same chromosomes as reported previously.

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