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We measured faecal corticosterone metabolites ( f CMs) in aquatic salamander  Necturus  to understand how inter-renal function changes throughout seasonal environmental changes and associated reproductive activity.  f CMs elevated with lower water temperatures and breeding activity. To overcome some challenges associated with faecal hormone collection/analysis, waterborne corticosterone ( w CM) was validated through ACTH challenge.

conservation physiology

Non-invasive methods to measure inter-renal function in aquatic salamanders—correlating fecal corticosterone to the environmental and physiologic conditions of captive Necturus