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Measures of stress hormone levels, specifically glucocorticoids and their metabolites, are increasingly used to index the stress burden experienced by wildlife. However, within-sample variation may affect interpretations of results. Here we quantified within-sample variation in faecal cortisol metabolite concentrations and conducted a power analysis to demonstrate sample-size requirements for detecting meaningful differences in stress hormone levels in wildlife populations following an environmental perturbation.

Within-sample variation in snowshoe hare faecal glucocorticoid metabolite measurements