Open AccessDetection of oocyte perivitelline membrane-bound sperm: a tool for avian collection management
Author(s) -
Kaitlin Croyle,
Barbara Durrant,
Thomas G. Jensen
Publication year - 2015
Publication title -
conservation physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.942
H-Index - 37
ISSN - 2051-1434
DOI - 10.1093/conphys/cou060
Subject(s) - sperm , biology , fertility , zoology , seasonal breeder , andrology , oocyte , mating , evolutionary biology , ecology , embryo , genetics , population , medicine , demography , sociology
This study evaluated the use of nuclear staining and PCR to detect the presence of sperm on avian oocyte membranes following incubation, room temperature and 4oC storage, and bacterial infection. The technique can be used as part of the reproductive evaluation process and for managing birds in captive breeding facilities.
Accelerating Research
Robert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom
Address
John Eccles HouseRobert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom