Open AccessUse of Macrorestriction Analysis to Demonstrate Interhospital Spread of Multiresistant Acinetobacter baumannii in Sao Paulo, Brazil
Author(s) -
Hélio S. Sader,
C. F. Mendes,
Antônio Carlos Campos Pignatari,
Michael A. Pfaller
Publication year - 1996
Publication title -
clinical infectious diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.44
H-Index - 336
eISSN - 1537-6591
pISSN - 1058-4838
DOI - 10.1093/clinids/23.3.631
Subject(s) - acinetobacter baumannii , sulbactam , pulsed field gel electrophoresis , microbiology and biotechnology , amikacin , ampicillin , broth microdilution , medicine , acinetobacter , polymyxin b , colistin , cephalosporin , antibiotics , biology , antibiotic resistance , minimum inhibitory concentration , bacteria , genotype , pseudomonas aeruginosa , imipenem , biochemistry , genetics , gene
We evaluated the spread of Acinetobacter baumannii strains among three hospitals located in São Paulo, Brazil. A total of 46 isolates, which were typed by chromosomal DNA analysis with use of pulsed field gel electrophoresis (PFGE), were tested for susceptibility to the fluoroquinolones, carbapenems, aminoglycosides (amikacin), cephalosporins, polymyxin B, and ampicillin/sulbactam by means of the broth microdilution method, disk diffusion, and the E-test. Isolates with an identical PFGE pattern (pattern B) that were susceptible only to carbapenems, polymyxin B, and ampicillin/ sulbactam were recovered in all three hospitals. In addition, isolates with PFGE pattern A that were susceptible only to polymyxin B and ampicillin/sulbactam were recovered in hospitals 1 and 2. The results of our study strongly suggest the interhospital transmission of multiresistant epidemic strains of A. baumannii in São Paulo. Once in the hospital, these strains can disseminate and cause outbreaks with devastating consequences.
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