Use of Restriction Endonuclease Analysis of Plasmids and Pulsed-Field Gel Electrophoresis to Investigate Outbreaks of Methicillin-Resistant Staphylococcus aureus Infection | Zendy

P. Y.-F. Liu | Zendy; ZhiYuan Shi | Zendy; Y J Lau | Zendy; Bor-Shen Hu | Zendy; Jainn-Ming Shyr | Zendy; Wei-Lin Tsai | Zendy; YuHui Lin | Zendy; C.-Y. Tseng | Zendy

Open Access

Use of Restriction Endonuclease Analysis of Plasmids and Pulsed-Field Gel Electrophoresis to Investigate Outbreaks of Methicillin-Resistant Staphylococcus aureus Infection

Author(s) -

P. Y.-F. Liu,

ZhiYuan Shi,

Y J Lau,

Bor-Shen Hu,

Jainn-Ming Shyr,

Wei-Lin Tsai,

YuHui Lin,

C.-Y. Tseng

Publication year - 1996

Publication title -

clinical infectious diseases

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.44

H-Index - 336

eISSN - 1537-6591

pISSN - 1058-4838

DOI - 10.1093/clinids/22.1.86

Subject(s) - pulsed field gel electrophoresis , staphylococcus aureus , medicine , outbreak , microbiology and biotechnology , restriction enzyme , typing , plasmid , micrococcaceae , infection control , methicillin resistant staphylococcus aureus , virology , antibacterial agent , biology , bacteria , surgery , antibiotics , genotype , dna , genetics , gene

We used restriction endonuclease analysis of plasmids (REAP) and pulsed-field gel electrophoresis (PFGE) to investigate clusterings of methicillin-resistant Staphylococcus aureus (MRSA) infections in our orthopedic unit, neurosurgery unit, intensive care unit, and burn unit. Fourteen different strain types were identified by REAP and 10 different strain types were identified by PFGE among 25 MRSA isolates collected during these incidents of infection. Though neither technique was clearly superior to the other for typing MRSA isolates, REAP is recommended as a relatively simple and reproducible technique for the preliminary investigation of MRSA infection outbreaks in clinical settings.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research