Coupled-Column Liquid Chromatographic Analysis of Catecholamines, Serotonin, and Metabolites in Human Urine | Zendy

Torsten Panholzer | Zendy; J Beyer | Zendy; K. Lichtwald | Zendy

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Coupled-Column Liquid Chromatographic Analysis of Catecholamines, Serotonin, and Metabolites in Human Urine

Author(s) -

Torsten Panholzer,

J Beyer,

K. Lichtwald

Publication year - 1999

Publication title -

clinical chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.705

H-Index - 218

eISSN - 1530-8561

pISSN - 0009-9147

DOI - 10.1093/clinchem/45.2.262

Subject(s) - chromatography , chemistry , normetanephrine , homovanillic acid , metanephrine , elution , analyte , high performance liquid chromatography , urine , serotonin , matrix (chemical analysis) , biochemistry , receptor

A column-switching HPLC system was utilized for the simultaneous determination of epinephrine, norepinephrine, dopamine, serotonin, and their metabolites metanephrine, normetanephrine, 3, 4-dihydroxyphenylacetic acid, homovanillic acid, and 5-hydroxyindoleacetic acid in human urine. The sample was injected directly onto a C18-alkyl-diol silica precolumn, which separated the analytes from matrix. The analytes were eluted from the precolumn onto the analytical column by the use of column-switching techniques and were then separated on the analytical column by means of ion-pair reversed-phase HPLC. The analytes were then oxidized to the corresponding quinones and converted into fluorescent derivatives by reaction with meso-1,2-diphenylethylenediamine.

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