Open AccessPreanalytical Variables Affecting the Quantification of Fatty Acid Ethyl Esters in Plasma and Serum Samples
Author(s) -
Britt L. Soderberg,
Ewa Sicińska,
Emily Blodget,
Joanne E. CluetteBrown,
Paolo M. Suter,
Theresa Schuppisser,
W Vetter,
Michael Laposata
Publication year - 1999
Publication title -
clinical chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.705
H-Index - 218
eISSN - 1530-8561
pISSN - 0009-9147
DOI - 10.1093/clinchem/45.12.2183
Subject(s) - chemistry , ethanol , chromatography , triglyceride , fatty acid , blood plasma , medicine , biochemistry , cholesterol
BACKGROUNDFatty acid ethyl esters (FAEEs) are cytotoxic nonoxidative ethanol metabolites produced by esterification of fatty acids and ethanol. FAEEs are detectable in blood up to 24 h after ethanol consumption. The objective of this study was to assess the impact of gender, serum or plasma triglyceride concentration, time and temperature of specimen storage, type of alcoholic beverage ingested, and the rate of ethanol consumption on FAEE concentrations in plasma or serum.METHODSFor some studies, subject were recruited volunteers; in others, residual blood samples after ethanol quantification were used. FAEEs were isolated by solid-phase extraction and quantified by gas chromatography-mass spectrometry.RESULTSFor weight-adjusted amounts of ethanol intake, FAEE concentrations were twofold greater for men than women (P /=24 h. The type of alcoholic beverage and rate of consumption did not affect FAEE concentrations.CONCLUSIONThese studies advance plasma and serum FAEE measurements closer to implementation as a clinical test for ethanol intake.
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