Open AccessHeteroduplex generator in analysis of Rh blood group alleles
Author(s) -
Jay Stoerker,
C Hurwitz,
N C Rose,
Leslie E. Silberstein,
W. Edward Highsmith
Publication year - 1996
Publication title -
clinical chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.705
H-Index - 218
eISSN - 1530-8561
pISSN - 0009-9147
DOI - 10.1093/clinchem/42.3.356
Subject(s) - heteroduplex , rh blood group system , allele , locus (genetics) , genetics , biology , microbiology and biotechnology , gene , antibody
We describe the use of heteroduplex analysis to enhance the resolution of different rhesus-derived (Rh) isotypes. Heteroduplex analysis of different domains of the Rh D and Rh CE loci can be performed to diagnose a variety of blood group incompatibilities. One application of this technique is the ability to test for fetal-maternal blood group incompatibilities during pregnancy. Several new serotype-specific sequence variations were discovered in the mapping of the Rh locus, and used in the construction of artificial heteroduplex generators (HGs). HGs facilitate the resolution of the Rh isotypes by electrophoretic methods.
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