Open AccessA Confirmed Case of SARS-CoV-2 Pneumonia With Negative Routine Reverse Transcriptase–Polymerase Chain Reaction and Virus Variation in Guangzhou, China
Author(s) -
Zhengtu Li,
Yinhu Li,
Lingdan Chen,
Shaoqiang Li,
Le Yu,
Airu Zhu,
Feng Yang,
Jiang Qian,
Liyan Chen,
Jincun Zhao,
Wenju Lu,
Nanshan Zhong,
Feng Ye
Publication year - 2020
Publication title -
clinical infectious diseases
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.44
H-Index - 336
eISSN - 1537-6591
pISSN - 1058-4838
DOI - 10.1093/cid/ciaa941
Subject(s) - polymerase chain reaction , virology , medicine , pneumonia , reverse transcriptase , virus , reverse transcription polymerase chain reaction , covid-19 , antibody , real time polymerase chain reaction , coronavirus , gene , immunology , biology , disease , infectious disease (medical specialty) , pathology , messenger rna , genetics
Background Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pneumonia is a newly recognized disease, and its diagnosis is primarily confirmed by routine reverse transcriptase -polymerase chain reaction (RT-PCR) detection of SARS-CoV-2. Methods However, we report a confirmed case of SARS-CoV-2 pneumonia with a negative routine RT-PCR. Results This case was finally diagnosed by nanopore sequencing combined with antibody of SARS-CoV-2. Simultaneously, the ORF and NP gene variations of SARS-CoV-2 were found. Conclusions This case highlighted that false-negative results could be present in routine RT-PCR diagnosis, especially with virus variation. Currently, nanopore pathogen sequencing and antibody detection have been found to be effective in clinical diagnosis.
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