Open AccessStudy on the Alkaloids in Tibetan MedicineAconitum pendulumBusch by HPLC–MSnCombined with Column Chromatography
Author(s) -
Beibei Wang,
Jie Dong,
Jiaojiao Ji,
Yuan Jiang,
Jiali Wang,
Jiarui Wu,
Peng Tan,
Yonggang Liu
Publication year - 2016
Publication title -
journal of chromatographic science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.362
H-Index - 56
eISSN - 1945-239X
pISSN - 0021-9665
DOI - 10.1093/chromsci/bmw002
Subject(s) - chemistry , chromatography , aconitum , high performance liquid chromatography , mass spectrometry , fragmentation (computing) , alkaloid , stereochemistry , computer science , operating system
A rapid, convenient and effective identification method of alkaloids was established and an attempt on isolating and analyzing the alkaloids in Aconitum pendulum Busch was conducted successfully. In this article, four high-content components including deoxyaconitine, benzoylaconine, aconine and neoline were isolated by using column chromatography. HPLC-MS(n)was employed to deduce the regulations of fragmentation of diterpenoid alkaloids which displayed a characteristic behavior of loss of CO(28u), CH3COOH(60u), CH3OH(32u), H2O(18u) and C6H5COOH(122u). Then, according to fragmentation regulation of mass spectrometry, 42 alkaloids were found inA. pendulum Among them, 38 compounds were identified and 29 alkaloids were reported for the first time for this herb. Therefore, this means that HPLC-MS(n)combined with column chromatography could work as an effective and reliable tool for rapid identification of the chemical components of herbal medicine.
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