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Three stereoselective HPLC methods have been developed for the chiral separation of the enantiomers of three intermediates in the preparation of (+)-tanikolide. The enantiomers were separated on Chiralpak IC (250 × 4.6 mm, 5 μm) in normal phase HPLC. Three intermediates were all baseline separated ( R  S  = 2.84, 2.58 and 3.58, respectively). By comparing the chromatograms of racemates and single enantiomers of the three intermediates, the  e.e.  values of the single enantiomers were determined and calculated.

Enantioseparation of Three Important Intermediates of Tanikolide with Immobilized Cellulose Chiral Stationary Phase