Open AccessEnantioseparation of Three Important Intermediates of Tanikolide with Immobilized Cellulose Chiral Stationary Phase
Author(s) -
Jie Zhou,
Qizhen Du,
S. J. Zhao,
Fei Sun,
Xinyu Li,
Zhenzhong Zhang
Publication year - 2014
Publication title -
journal of chromatographic science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.362
H-Index - 56
eISSN - 1945-239X
pISSN - 0021-9665
DOI - 10.1093/chromsci/bmu158
Subject(s) - enantiomer , chemistry , chromatography , chiral stationary phase , high performance liquid chromatography , stereoselectivity , cellulose , chiral column chromatography , stereoisomerism , chiral derivatizing agent , phase (matter) , organic chemistry , molecule , catalysis
Three stereoselective HPLC methods have been developed for the chiral separation of the enantiomers of three intermediates in the preparation of (+)-tanikolide. The enantiomers were separated on Chiralpak IC (250 × 4.6 mm, 5 μm) in normal phase HPLC. Three intermediates were all baseline separated (RS = 2.84, 2.58 and 3.58, respectively). By comparing the chromatograms of racemates and single enantiomers of the three intermediates, the e.e. values of the single enantiomers were determined and calculated.