Open AccessScreening and Identification of Multi-Components in Re Du Ning Injections Using LC/TOF-MS Coupled with UV-Irradiation
Author(s) -
Lingzhi Li,
Zhenzhong Wang,
Ying Peng,
Xiaohuan Fu,
Yongxiang Wang,
Wei Xiao,
Shaojiang Song
Publication year - 2014
Publication title -
journal of chromatographic science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.362
H-Index - 56
eISSN - 1945-239X
pISSN - 0021-9665
DOI - 10.1093/chromsci/bmu125
Subject(s) - chemistry , chromatography , mass spectrometry , gardenia jasminoides , high performance liquid chromatography , quadrupole time of flight , glycoside , time of flight mass spectrometry , tandem mass spectrometry , stereochemistry , organic chemistry , ionization , medicine , ion , alternative medicine , pathology
A high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry-UV-irradiation (HPLC-QTOF-MS-UV) method was established for rapid separation and structural identification of the constituents in Re Du Ning Injections (RDNI). A total of 20 potentially bioactive compounds including 10 caffeoylquinic acids and 10 iridoid glycosides were identified or tentatively characterized in RDNI by comparing their retention times and MS spectra with those of authentic standards or literature data. In particular, UV-irradiation was employed in the identification of the cis/trans isomers of caffeoylquinic acids. Furthermore, each compound was assigned to the individual raw materials (Artemisia annua L., Lonicera japonica Thunb. or Gardenia jasminoides Ellis) present in RDNI. This is the first time that an HPLC-QTOF-MS-UV analytical method has been used for the identification of caffeoylquinic acids in RDNI.
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