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A novel cyclodextrin (CD) derivative mono(6(A)-N-allylamino-6(A)-deoxy)per-3-chlorine-4-methyl-phenylcarbamoylated-β-CD was synthesized and chemically immobilized onto the surface of γ-mercaptopropyl-functionalized silica gel step by step. The products were all purified and characterized and then got a substance with definitional structure. This chiral stationary phase (CSP) of 3-chlorine-4-methyl-phenylcarbamoylated-β-CD bonded on silica gel exhibited excellent separation capability for several chiral compounds in high-performance liquid chromatography. In the enantiomeric separations of five racemates with a mixture of methanol and aqueous KH2PO4 buffer as the mobile phase, this CSP presented good chiral recognition performance.

journal of chromatographic science

Preparation and Enantioseparation Characteristics of a Novel β-Cyclodextrin Derivative Chiral Stationary Phase in High-Performance Liquid Chromatography