Quantification of Sesquiterpene Lactones in Parthenium hyterophorous by Normal-Phase HPLC | Zendy

Renu Chib | Zendy; Bhahwal Ali Shah | Zendy; Samar S. Andotra | Zendy; Vikram Bharadwaj | Zendy; Rajinder K. Gupta | Zendy; Subhash C. Taneja | Zendy; Ravi K. Khajuria | Zendy

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Quantification of Sesquiterpene Lactones in Parthenium hyterophorous by Normal-Phase HPLC

Author(s) -

Renu Chib,

Bhahwal Ali Shah,

Samar S. Andotra,

Vikram Bharadwaj,

Rajinder K. Gupta,

Subhash C. Taneja,

Ravi K. Khajuria

Publication year - 2013

Publication title -

journal of chromatographic science

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.362

H-Index - 56

eISSN - 1945-239X

pISSN - 0021-9665

DOI - 10.1093/chromsci/bms195

Subject(s) - chromatography , chemistry , sesquiterpene , parthenium , hexane , isopropyl alcohol , high performance liquid chromatography , silica gel , isopropyl , calibration curve , detection limit , organic chemistry , botany , weed , biology

This paper describes the development of a normal-phase liquid chromatography ultraviolet-diode array detection method for the simultaneous quantification of parthenin and coronopilin in the leaves and flowers of Parthenium hysterophorous. The compounds were analyzed on a Merck Si60 silica column (5 µm, 250 × 4 mm) using an isocratic 15:85 mixture of isopropyl alcohol and hexane. The calibration curves resulting from the reference compounds in the concentration range of 200-2,000 ng exhibited acceptable linearity (r > 0.999). The method was developed to study the levels of parthenin and coronopilin in the leaves and flowers of P. hysterophorous collected during different seasons, and the method was validated by analyzing the spiked samples.

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