Analysis of Glyoxal and Related Substances by Means of High-Performance Liquid Chromatography with Refractive Index Detection | Zendy

Zhiyong Zhang | Zendy; Zhao Di-shun | Zendy; Baoyun Xu | Zendy

Open Access

Analysis of Glyoxal and Related Substances by Means of High-Performance Liquid Chromatography with Refractive Index Detection

Author(s) -

Zhiyong Zhang,

Zhao Di-shun,

Baoyun Xu

Publication year - 2012

Publication title -

journal of chromatographic science

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.362

H-Index - 56

eISSN - 1945-239X

pISSN - 0021-9665

DOI - 10.1093/chromsci/bms186

Subject(s) - chemistry , glyoxal , chromatography , formic acid , acetaldehyde , oxalic acid , high performance liquid chromatography , acetic acid , aqueous solution , formaldehyde , detection limit , glycolic acid , ethanol , organic chemistry , genetics , lactic acid , biology , bacteria

A simple and rapid method is described for the analysis of glyoxal and related substances by high-performance liquid chromatography with a refractive index detector. The following chromatographic conditions were adopted: Aminex HPX-87H column, mobile phase consisting of 0.01N H2SO4, flow rate of 0.8 mL/min and temperature of 65°C. The application of the analytical technique developed in this study demonstrated that the aqueous reaction mixture produced by the oxidation of acetaldehyde with HNO3 was composed of glyoxal, acetaldehyde, acetic acid, formic acid, glyoxylic acid, oxalic acid, butanedione and glycolic acid. The method was validated by evaluating analytical parameters such as linearity, limits of detection and quantification, precision, recovery and robustness. The proposed methodology was successfully applied to the production of glyoxal.

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