Sensitive HPLC--ESI-MS Method for the Determination of Tiotropium in Human Plasma | Zendy

Li Ding | Zendy; Wen Tan | Zendy; Y. Zhang | Zendy; Jiying Shen | Zendy; Zhou Zhang | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Sensitive HPLC--ESI-MS Method for the Determination of Tiotropium in Human Plasma

Author(s) -

Li Ding,

Wen Tan,

Y. Zhang,

Jiying Shen,

Zhou Zhang

Publication year - 2008

Publication title -

journal of chromatographic science

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.362

H-Index - 56

eISSN - 1945-239X

pISSN - 0021-9665

DOI - 10.1093/chromsci/46.5.445

Subject(s) - chemistry , chromatography , electrospray ionization , high performance liquid chromatography , ammonium acetate , calibration curve , mass spectrometry , pharmacokinetics , electrospray , ethyl acetate , detection limit , pharmacology , medicine

A sensitive high-performance liquid chromatography-electrospray ionization-mass spectrometry (HPLC-ESI-MS) assay is established for the determination of tiotropium in human plasma using benzyltriethylammonium chloride as the internal standard (IS). After being treated with C(18) cartridges, plasma samples are separated by HPLC on a reversed-phase C(18) column with a mobile phase of 40mM ammonium acetate buffer-methanol (56:44, v/v). Tiotropium is determined in a single-quadrupole MS. HPLC-ESI-MS is performed in the selected ion monitoring mode using target ions at m/z 392.0 for tiotropium and m/z 192.3 for the IS. The calibration curve is linear over the range 1.5-30 pg/mL. The intra- and inter-assay variability values are less than 10.1% and 13.6%, respectively. The mean plasma extraction recovery of tiotropium is 92.3 +/- 5.0%. The method has been successfully applied to studying the pharmacokinetics of tiotropium in healthy Chinese volunteers.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research