Fluconazol Method Validation by RP-HPLC for Determination in Biological Skin Matrices | Zendy

Alessandra C. Ayub | Zendy; Cristina Duarte ViannaSoares | Zendy; Lucas Antônio Miranda Ferreira | Zendy

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Fluconazol Method Validation by RP-HPLC for Determination in Biological Skin Matrices

Author(s) -

Alessandra C. Ayub,

Cristina Duarte ViannaSoares,

Lucas Antônio Miranda Ferreira

Publication year - 2007

Publication title -

journal of chromatographic science

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.362

H-Index - 56

eISSN - 1945-239X

pISSN - 0021-9665

DOI - 10.1093/chromsci/45.5.286

Subject(s) - chemistry , chromatography , fluconazole , permeation , pharmacokinetics , antifungal , high performance liquid chromatography , drug , in vitro , pharmacology , dermatology , medicine , membrane , biochemistry

The bis-triazole antifungal fluconazole (FCZ) is used in the systemic treatment of superficial mycoses. The inconvenience of drug interactions and incidence of adverse reactions occurs in approximately 16% of patients, despite several advantages against systemic fungal infections. Because its pharmacokinetics profile is favorable to cutaneous accumulation, it presents a prominent importance in the treatment of superficial mycoses. This study shows FCZ method validation by reversed-phase high-performance liquid chromatography in the linear range of 2 to 32 microg/mL, which suitable for application in biological matrices after topical permeation studies. The method is tested in simulated FCZ alcoholic solution applied to skin extracts after in vitro permeation studies using Franz cells. Recovery shows good results (in the range 75.0% +/- 4.1% to 82.0% +/- 6.6%) regarding the biological matrices.

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