Application of Curve Fitting in Thin-Layer Chromatography-Flame Ionization Detection Analysis of the Carbohydrate Fraction in Marine Mucilage and Marine Snow Samples from Italian Seas | Zendy

M. Meccozi | Zendy; Zsuzsanna Pápai | Zendy

Open Access

Application of Curve Fitting in Thin-Layer Chromatography-Flame Ionization Detection Analysis of the Carbohydrate Fraction in Marine Mucilage and Marine Snow Samples from Italian Seas

Author(s) -

M. Meccozi,

Zsuzsanna Pápai

Publication year - 2004

Publication title -

journal of chromatographic science

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.362

H-Index - 56

eISSN - 1945-239X

pISSN - 0021-9665

DOI - 10.1093/chromsci/42.5.268

Subject(s) - chemistry , chromatography , derivatization , fraction (chemistry) , gas chromatography , mucilage , marine snow , flame ionization detector , monosaccharide , snow , deconvolution , carbohydrate , high performance liquid chromatography , geology , organic chemistry , botany , oceanography , algorithm , geomorphology , water column , computer science , biology

This paper presents a thin-layer chromatographic-flame ionization detection (TLC-FID) procedure to characterize the carbohydrate fraction of marine mucilage and marine snow samples from the Italian Seas. The identification of the different carbohydrate subfractions is supported by the application of a deconvolution procedure based on a new mathematical function for describing chromatographic peaks and enhancing their resolution. The joint-approach TLC-FID analysis and deconvolution procedure allows for the characterization of the carbohydrate fraction of the marine samples in a single step without using the different derivatization procedures requested by the most common gas chromatography and high-performance liquid chromatographic methods for carbohydrate analysis. In fact, the results obtained by the TLC-FID procedure show that different neutral, uronic acid, and aminosugar subfractions can be present simultaneously in these samples. Moreover, the results support some hypotheses about the causes of the presence of mucilages in the Italian Seas.

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