Chromatographic Analysis of Sagebrush Monoterpenes in Blood Plasma | Zendy

Bruce A. Kimball | Zendy; Luthando Dziba | Zendy; John J. Johnston | Zendy; F D Provenza | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Chromatographic Analysis of Sagebrush Monoterpenes in Blood Plasma

Author(s) -

Bruce A. Kimball,

Luthando Dziba,

John J. Johnston,

F D Provenza

Publication year - 2004

Publication title -

journal of chromatographic science

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.362

H-Index - 56

eISSN - 1945-239X

pISSN - 0021-9665

DOI - 10.1093/chromsci/42.5.245

Subject(s) - chemistry , chromatography , analyte , extraction (chemistry) , solid phase extraction , gas chromatography , sample preparation , flame ionization detector , quantitative analysis (chemistry) , pharmacokinetics , medicine

An analytical method is required for the analysis of monoterpenes in animal plasma to support a pharmacokinetic study. Monoterpenes common to sagebrush are extracted from sheep plasma by employing solid-phase extraction (SPE), followed by analysis of the extracts by gas chromatography with flame ionization detection. The analytes are quantitated versus an external standard and by comparison with a surrogate standard added to the sample prior to extraction. In addition to comparing the two quantitative methods, the storage stability of the analytes in plasma and SPE columns is evaluated. Both methods employed for quantitation yield precision suitable for pharmacokinetic studies. However, determination of monoterpenes residues versus external standards produces improved accuracy as compared with use of the surrogate standard. Some analyte loss is observed from plasma samples stored for five weeks at -12 degrees C. However, storage of extracts on the SPE columns affords excellent stability.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research