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Simultaneous Determination of Seven Compounds in Snow Lotus Herb Using High-Performance Liquid Chromatography
Author(s) -
Xiaodong Zhang,
Miao Yu,
Jie Chen
Publication year - 2003
Publication title -
journal of chromatographic science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.362
H-Index - 56
eISSN - 1945-239X
pISSN - 0021-9665
DOI - 10.1093/chromsci/41.5.241
Subject(s) - chemistry , chromatography , rutin , luteolin , apigenin , high performance liquid chromatography , herb , reversed phase chromatography , flavonoid , traditional medicine , medicinal herbs , organic chemistry , antioxidant , medicine
A reversed-phase high-performance liquid chromatographic method is developed for the quantitative determination of seven components of Snow Lotus Herb (Saussurea tridactyla Sch.-Bip.ex Hook.f.): umbelliferonglucoside, luteolin-7-O-beta-D-glucoside, rutin, apigenin-7-O-beta-D-glucoside, kaempferol-3-O-beta-D-glucoside, apigenin-7-O-beta-D-rutinoside, and luteolin. Samples are analyzed by means of a reverse-phase column (Diamonsil C(18)) using methanol and water under gradient conditions as the mobile phase for 60 min. This method offers selectivity, accuracy, precision, linearity, and ruggedness, as well as efficiency and ease.

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