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The development of next generation sequencing technology provides an efficient and powerful approach to rare variant detection. To identify genetic variations, the essential question is how to quantity the sequencing error rate in the data. Because of the advantage of easy implementation and the ability to integrate data from different sources, the empirical Bayes method is popularly employed to estimate the sequencing error rate for SNP detection.

An empirical Bayes method for genotyping and SNP detection using multi-sample next-generation sequencing data