Cas-Designer: a web-based tool for choice of CRISPR-Cas9 target sites | Zendy

Jeongbin Park | Zendy; Sangsu Bae | Zendy; JinSoo Kim | Zendy

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Cas-Designer: a web-based tool for choice of CRISPR-Cas9 target sites

Author(s) -

Jeongbin Park,

Sangsu Bae,

JinSoo Kim

Publication year - 2015

Publication title -

bioinformatics

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.599

H-Index - 390

eISSN - 1367-4811

pISSN - 1367-4803

DOI - 10.1093/bioinformatics/btv537

Subject(s) - crispr , computer science , cas9 , genome editing , frame (networking) , guide rna , table (database) , computational biology , world wide web , gene , genetics , data mining , biology , telecommunications

We present Cas-Designer, a user-friendly program to aid researchers in choosing appropriate target sites in a gene of interest for type II CRISPR/Cas-derived RNA-guided endonucleases, which are now widely used for biomedical research and biotechnology. Cas-Designer rapidly provides the list of all possible guide RNA sequences in a given input DNA sequence and their potential off-target sites including bulge-type sites in a genome of choice. In addition, the program assigns an out-of-frame score to each target site to help users choose appropriate sites for gene knockout. Cas-Designer shows the results in an interactive table and provides user-friendly filter functions.

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