P-SAMS: a web site for plant artificial microRNA and synthetic trans-acting small interfering RNA design | Zendy

Noah Fahlgren | Zendy; Steven T. Hill | Zendy; James C. Carrington | Zendy; Alberto Carbonell | Zendy

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P-SAMS: a web site for plant artificial microRNA and synthetic trans-acting small interfering RNA design

Author(s) -

Noah Fahlgren,

Steven T. Hill,

James C. Carrington,

Alberto Carbonell

Publication year - 2015

Publication title -

bioinformatics

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.599

H-Index - 390

eISSN - 1367-4811

pISSN - 1367-4803

DOI - 10.1093/bioinformatics/btv534

Subject(s) - oligonucleotide , microrna , computational biology , rna , gene silencing , small rna , small interfering rna , computer science , biology , cloning (programming) , gene , genetics , programming language

The Plant Small RNA Maker Site (P-SAMS) is a web tool for the simple and automated design of artificial miRNAs (amiRNAs) and synthetic trans-acting small interfering RNAs (syn-tasiRNAs) for efficient and specific targeted gene silencing in plants. P-SAMS includes two applications, P-SAMS amiRNA Designer and P-SAMS syn-tasiRNA Designer. The navigation through both applications is wizard-assisted, and the job runtime is relatively short. Both applications output the sequence of designed small RNA(s), and the sequence of the two oligonucleotides required for cloning into 'B/c' compatible vectors.

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