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The 14-3-3 family of phosphoprotein-binding proteins regulates many cellular processes by docking onto pairs of phosphorylated Ser and Thr residues in a constellation of intracellular targets. Therefore, there is a pressing need to develop new prediction methods that use an updated set of 14-3-3-binding motifs for the identification of new 14-3-3 targets and to prioritize the downstream analysis of &gt;2000 potential interactors identified in high-throughput experiments.

14-3-3-Pred: improved methods to predict 14-3-3-binding phosphopeptides