Using isoelectric point to determine the pH for initial protein crystallization trials | Zendy

Jobie Kirkwood | Zendy; David Hargreaves | Zendy; Simon O’Keefe | Zendy; Julie Wilson | Zendy

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Using isoelectric point to determine the pH for initial protein crystallization trials

Author(s) -

Jobie Kirkwood,

David Hargreaves,

Simon O’Keefe,

Julie Wilson

Publication year - 2015

Publication title -

bioinformatics

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.599

H-Index - 390

eISSN - 1367-4811

pISSN - 1367-4803

DOI - 10.1093/bioinformatics/btv011

Subject(s) - isoelectric point , crystallization , isoelectric focusing , point (geometry) , computer science , protein crystallization , chromatography , chemistry , mathematics , biochemistry , geometry , enzyme , organic chemistry

The identification of suitable conditions for crystallization is a rate-limiting step in protein structure determination. The pH of an experiment is an important parameter and has the potential to be used in data-mining studies to help reduce the number of crystallization trials required. However, the pH is usually recorded as that of the buffer solution, which can be highly inaccurate.

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