Open AccessDevelopment of a domain-specific genetic language to design Chlamydomonas reinhardtii expression vectors
Author(s) -
Mandy Wilson,
Sakiko Okumoto,
Laura Adam,
Jean Peccoud
Publication year - 2013
Publication title -
bioinformatics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.599
H-Index - 390
eISSN - 1367-4811
pISSN - 1367-4803
DOI - 10.1093/bioinformatics/btt646
Subject(s) - chlamydomonas reinhardtii , computer science , context (archaeology) , programming language , synthetic biology , expression cassette , computational biology , domain specific language , domain (mathematical analysis) , source code , code generation , biology , vector (molecular biology) , gene , genetics , mathematics , paleontology , mathematical analysis , computer security , key (lock) , mutant , recombinant dna
Expression vectors used in different biotechnology applications are designed with domain-specific rules. For instance, promoters, origins of replication or homologous recombination sites are host-specific. Similarly, chromosomal integration or viral delivery of an expression cassette imposes specific structural constraints. As de novo gene synthesis and synthetic biology methods permeate many biotechnology specialties, the design of application-specific expression vectors becomes the new norm. In this context, it is desirable to formalize vector design strategies applicable in different domains.