TACTICS, an interactive platform for customized high-content bioimaging analysis | Zendy

Raz Shimoni | Zendy; Kim Pham | Zendy; Mohammed Yassin | Zendy; Miṅ Gu | Zendy; Sarah M. Russell | Zendy

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TACTICS, an interactive platform for customized high-content bioimaging analysis

Author(s) -

Raz Shimoni,

Kim Pham,

Mohammed Yassin,

Miṅ Gu,

Sarah M. Russell

Publication year - 2013

Publication title -

bioinformatics

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.599

H-Index - 390

eISSN - 1367-4811

pISSN - 1367-4803

DOI - 10.1093/bioinformatics/btt035

Subject(s) - toolbox , computer science , software , modular design , matlab , graphical user interface , source code , scripting language , interface (matter) , user interface , image processing , data mining , sample (material) , computer graphics (images) , human–computer interaction , multimedia , database , information retrieval , image (mathematics) , computer vision , programming language , operating system , bubble , maximum bubble pressure method , chemistry , chromatography

We describe a modular MATLAB® Toolbox named TACTICS for time-lapse image analysis that meets several requirements not generally offered by currently available software packages: (i) the ability to assess quality of extracted imaging information by directly linking data end points to the original position, (ii) massively parallel analysis of each parameter, for flow cytometry-like assessment of possible relationships between parameters within sub-populations of the images, (iii) options for user control of the tracking such as an interface to restrict the analysis region, (iv) manual correction of automated processes and (v) user interfaces for post-tracking analysis that is linked to the original images, including options to view cell pedigrees and normalized polarization ratios based on fluorescence ratiometric measurements.

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