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pIRS: Profile-based Illumina pair-end reads simulator
Author(s) -
Xuesong Hu,
Jianying Yuan,
Yujian Shi,
Jianliang Lu,
Binghang Liu,
Zhenyu Li,
Yanxiang Chen,
Desheng Mu,
Hao Zhang,
Nan Li,
Zhen Yue,
Fan Bai,
Heng Li,
Wei Fan
Publication year - 2012
Publication title -
bioinformatics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.599
H-Index - 390
eISSN - 1367-4811
pISSN - 1367-4803
DOI - 10.1093/bioinformatics/bts187
Subject(s) - perl , computer science , software , file transfer protocol , sequence assembly , illumina dye sequencing , data mining , reference genome , dna sequencing , computational biology , biology , the internet , genetics , world wide web , operating system , gene , gene expression , transcriptome
The next-generation high-throughput sequencing technologies, especially from Illumina, have been widely used in re-sequencing and de novo assembly studies. However, there is no existing software that can simulate Illumina reads with real error and quality distributions and coverage bias yet, which is very useful in relevant software development and study designing of sequencing projects.

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