Expression ratio evaluation in two-colour microarray experiments is significantly improved by correcting image misalignment | Zendy

Thomas LiPing Tang | Zendy; Nicolas François | Zendy; Annie Glatigny | Zendy; Nicolas Agier | Zendy; Marie-Hélène Mucchielli | Zendy; Lawrence P. Aggerbeck | Zendy; Hervé Delacroix | Zendy

Open Access

Expression ratio evaluation in two-colour microarray experiments is significantly improved by correcting image misalignment

Author(s) -

Thomas LiPing Tang,

Nicolas François,

Annie Glatigny,

Nicolas Agier,

Marie-Hélène Mucchielli,

Lawrence P. Aggerbeck,

Hervé Delacroix

Publication year - 2007

Publication title -

bioinformatics

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.599

H-Index - 390

eISSN - 1367-4811

pISSN - 1367-4803

DOI - 10.1093/bioinformatics/btm399

Subject(s) - robustness (evolution) , estimator , computer science , artificial intelligence , dna microarray , pattern recognition (psychology) , image quality , interpolation (computer graphics) , image (mathematics) , correlation , computer vision , mathematics , statistics , gene expression , biology , gene , biochemistry , geometry

Two-colour microarrays are widely used to perform transcriptome analysis. In most cases, it appears that the 'red' and 'green' images resulting from the scan of a microarray slide are slightly shifted one with respect to the other. To increase the robustness of the measurement of the fluorescent emission intensities, multiple acquisitions with the same or different PMT gains can be used. In these cases, a systematic correction of image shift is required.

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