Open AccessCorrecting for gene-specific dye bias in DNA microarrays using the method of maximum likelihood
Author(s) -
Ryan Kelley,
Hoda Feizi,
Trey Ideker
Publication year - 2007
Publication title -
bioinformatics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.599
H-Index - 390
eISSN - 1367-4811
pISSN - 1367-4803
DOI - 10.1093/bioinformatics/btm347
Subject(s) - dna microarray , dna , computer science , maximum likelihood , computational biology , statistics , gene , genetics , biology , mathematics , gene expression
In two-color microarray experiments, well-known differences exist in the labeling and hybridization efficiency of Cy3 and Cy5 dyes. Previous reports have revealed that these differences can vary on a gene-by-gene basis, an effect termed gene-specific dye bias. If uncorrected, this bias can influence the determination of differentially expressed genes.
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