TRFMA: a web-based tool for terminal restriction fragment length polymorphism analysis based on molecular weight
Author(s) -
Yoshio Nakano,
Toru Takeshita,
Yoshihisa Yamashita
Publication year - 2006
Publication title -
bioinformatics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.599
H-Index - 390
eISSN - 1367-4811
pISSN - 1367-4803
DOI - 10.1093/bioinformatics/btl186
Subject(s) - xml , computer science , fragment (logic) , terminal restriction fragment length polymorphism , restriction fragment length polymorphism , the internet , web browser , web site , web server , world wide web , web application , database , biology , genetics , programming language , polymerase chain reaction , gene
TRFMA provides a Web environment for analyzing T-RFLP results based on molecular weights of the fragments, rather than the numbers of nucleotides, to increase accuracy. The 16S rRNA data are saved as an XML file containing around 650 sequences (light version) and a MySQL database containing around 50 000 sequences (full version), which are connected to Web server via PHP5 and manipulated on an Internet browser.
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