A calibration method for estimating absolute expression levels from microarray data | Zendy

Kristof Engelen | Zendy; Bart Naudts | Zendy; Bart De Moor | Zendy; Kathleen Marchal | Zendy

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A calibration method for estimating absolute expression levels from microarray data

Author(s) -

Kristof Engelen,

Bart Naudts,

Bart De Moor,

Kathleen Marchal

Publication year - 2006

Publication title -

bioinformatics

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.599

H-Index - 390

eISSN - 1367-4811

pISSN - 1367-4803

DOI - 10.1093/bioinformatics/btl068

Subject(s) - calibration , expression (computer science) , computer science , sample (material) , microarray analysis techniques , microarray , computational biology , data mining , statistics , gene expression , biology , mathematics , gene , genetics , chemistry , chromatography , programming language

We describe an approach to normalize spotted microarray data, based on a physically motivated calibration model. This model consists of two major components, describing the hybridization of target transcripts to their corresponding probes on the one hand, and the measurement of fluorescence from the hybridized, labeled target on the other hand. The model parameters and error distributions are estimated from external control spikes.

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