Evaluating putative chimeric sequences from PCR-amplified products | Zendy

Juan Miguel González Grau | Zendy; Johannes Zimmermann | Zendy; Cesáreo SáizJiménez | Zendy

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Evaluating putative chimeric sequences from PCR-amplified products

Author(s) -

Juan Miguel González Grau,

Johannes Zimmermann,

Cesáreo SáizJiménez

Publication year - 2004

Publication title -

bioinformatics

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.599

H-Index - 390

eISSN - 1367-4811

pISSN - 1367-4803

DOI - 10.1093/bioinformatics/bti008

Subject(s) - biology , computational biology , sequence (biology) , chimeric gene , genetics , gene , sequence analysis , chimera (genetics) , dna sequencing , gene expression

PCR amplification of highly homologous genes from complex DNA mixtures is known to generate a significant proportion of chimeric sequences. Ribosomal RNA genes are used for microbial species detection and identification in natural environments, and current assessments of microbial diversity are based on these sequences. Thus, chimeric sequences could lead to the discovery of non-existent microbial species and false diversity estimates.

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