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EC_oligos: automated and whole-genome primer design for exons within one or between two genomes
Author(s) -
Shaolin Liu,
Nicholas A. Tinker,
Stephen J. Molnar,
Diane E. Mather
Publication year - 2004
Publication title -
bioinformatics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.599
H-Index - 390
eISSN - 1367-4811
pISSN - 1367-4803
DOI - 10.1093/bioinformatics/bth413
Subject(s) - executable , oligonucleotide , genome , primer (cosmetics) , computational biology , exon , biology , file transfer protocol , genetics , computer science , sequence (biology) , dna , programming language , gene , world wide web , chemistry , the internet , organic chemistry
EC_oligos designs oligonucleotides (oligos) from exons of annotated genomic sequence information. It can automatically and rapidly select oligos that are conserved between two sets of sequence data, and can pair up oligos for use as PCR primers. It can do this on a whole-genome scale and according to user-defined criteria.

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