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UniFrag and GenomePrimer: selection of primers for genome-wide production of unique amplicons
Author(s) -
Sacha A. F. T. van Hijum,
Anne de Jong,
Girbe Buist,
Jan Kok,
Oscar P. Kuipers
Publication year - 2003
Publication title -
bioinformatics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.599
H-Index - 390
eISSN - 1367-4811
pISSN - 1367-4803
DOI - 10.1093/bioinformatics/btg203
Subject(s) - amplicon , primer (cosmetics) , computer science , genome , selection (genetic algorithm) , computational biology , dna sequencing , genomic information , biology , genetics , dna , polymerase chain reaction , artificial intelligence , gene , chemistry , organic chemistry
The complementary programs UniFrag and GenomePrimer were developed to provide a reliable high-throughput method to select the most unique regions within genomic DNA sequence(s) and design primers therein, involving minimal user intervention and maximum flexibility.

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