Differential expression in SAGE: accounting for normal between-library variation | Zendy

Keith Baggerly | Zendy; Li Deng | Zendy; Jeffrey S. Morris | Zendy; C. Marcelo Aldaz | Zendy

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Differential expression in SAGE: accounting for normal between-library variation

Author(s) -

Keith Baggerly,

Li Deng,

Jeffrey S. Morris,

C. Marcelo Aldaz

Publication year - 2003

Publication title -

bioinformatics

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.599

H-Index - 390

eISSN - 1367-4811

pISSN - 1367-4803

DOI - 10.1093/bioinformatics/btg173

Subject(s) - variation (astronomy) , statistic , sampling (signal processing) , sage , inference , computer science , statistics , differential (mechanical device) , expression (computer science) , mathematics , artificial intelligence , programming language , physics , astrophysics , nuclear physics , engineering , aerospace engineering , filter (signal processing) , computer vision

In contrasting levels of gene expression between groups of SAGE libraries, the libraries within each group are often combined and the counts for the tag of interest summed, and inference is made on the basis of these larger 'pseudolibraries'. While this captures the sampling variability inherent in the procedure, it fails to allow for normal variation in levels of the gene between individuals within the same group, and can consequently overstate the significance of the results. The effect is not slight: between-library variation can be hundreds of times the within-library variation.

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