RainbowSTORM: an open-source ImageJ plug-in for spectroscopic single-molecule localization microscopy (sSMLM) data analysis and image reconstruction | Zendy

Janel L. Davis | Zendy; Brian T. Soetikno | Zendy; Ki-Hee Song | Zendy; Yang Zhang | Zendy; Cheng Sun | Zendy; Hao F. Zhang | Zendy

Open Access

RainbowSTORM: an open-source ImageJ plug-in for spectroscopic single-molecule localization microscopy (sSMLM) data analysis and image reconstruction

Author(s) -

Janel L. Davis,

Brian T. Soetikno,

Ki-Hee Song,

Yang Zhang,

Cheng Sun,

Hao F. Zhang

Publication year - 2020

Publication title -

bioinformatics

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.599

H-Index - 390

eISSN - 1367-4811

pISSN - 1367-4803

DOI - 10.1093/bioinformatics/btaa635

Subject(s) - software , open source , open source software , visualization , computer science , plug in , java , fluorescence , microscopy , fluorescence microscope , computer graphics (images) , computer vision , artificial intelligence , operating system , physics , optics

Spectroscopic single-molecule localization microscopy (sSMLM) simultaneously captures the spatial locations and full spectra of stochastically emitting fluorescent single molecules. It provides an optical platform to develop new multimolecular and functional imaging capabilities. While several open-source software suites provide subdiffraction localization of fluorescent molecules, software suites for spectroscopic analysis of sSMLM data remain unavailable. RainbowSTORM is an open-source ImageJ/FIJI plug-in for end-to-end spectroscopic analysis and visualization for sSMLM images. RainbowSTORM allows users to calibrate, preview and quantitatively analyze emission spectra acquired using different reported sSMLM system designs and fluorescent labels.

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