Q-RT-PCR: data analysis software for measurement of gene expression by competitive RT-PCR | Zendy

Peter A. Doris | Zendy; Amanda Hayward-Lester | Zendy; Jon K. Hays | Zendy

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Q-RT-PCR: data analysis software for measurement of gene expression by competitive RT-PCR

Author(s) -

Peter A. Doris,

Amanda Hayward-Lester,

Jon K. Hays

Publication year - 1997

Publication title -

bioinformatics

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 3.599

H-Index - 390

eISSN - 1367-4811

pISSN - 1367-4803

DOI - 10.1093/bioinformatics/13.6.587

Subject(s) - software , microsoft excel , computer science , reverse transcription polymerase chain reaction , gene , gene expression , data mining , computational biology , operating system , biology , genetics

We have developed software to assist in the computation of gene expression from data obtained in competitive reverse transcription-polymerase chain reaction (RT-PCR). This report describes the mathematical basis of competitive RT-PCR and discusses the criteria which must be met to permit accurate estimations of gene expression to be obtained using this technique.

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