Experimental design for single-cell RNA sequencing | Zendy

Jeanette BaranGale | Zendy; Tamir Chandra | Zendy; Kristina Kirschner | Zendy

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Experimental design for single-cell RNA sequencing

Author(s) -

Jeanette BaranGale,

Tamir Chandra,

Kristina Kirschner

Publication year - 2017

Publication title -

briefings in functional genomics

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.22

H-Index - 67

eISSN - 2041-2647

pISSN - 2041-2649

DOI - 10.1093/bfgp/elx035

Subject(s) - biology , computational biology , rna , variety (cybernetics) , field (mathematics) , rna seq , computer science , gene , genetics , artificial intelligence , gene expression , transcriptome , mathematics , pure mathematics

Single-cell RNA sequencing (scRNA-seq) has opened new avenues for the characterization of heterogeneity in a large variety of cellular systems. As this is a relatively new technique, the field is fast evolving. Here, we discuss general considerations in experimental design and the two most popular approaches, plate-based Smart-Seq2 and microdroplet-based scRNA-seq at the example of 10x Chromium. We discuss advantages and disadvantages of both methods and point out major factors to consider in designing successful experiments.

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