Safe sequencing depth to estimate the intra-host heterogeneity of viruses | Zendy

Xianwen Ren | Zendy; Qi Jin | Zendy

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Safe sequencing depth to estimate the intra-host heterogeneity of viruses

Author(s) -

Xianwen Ren,

Qi Jin

Publication year - 2015

Publication title -

briefings in functional genomics

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.22

H-Index - 67

eISSN - 2041-2647

pISSN - 2041-2649

DOI - 10.1093/bfgp/elv039

Subject(s) - biology , massive parallel sequencing , genome , computational biology , sequence assembly , deep sequencing , host (biology) , dna sequencing , genetics , whole genome sequencing , gene , transcriptome , gene expression

Massively parallel sequencing allows efficient determination of genomic sequences and intra-host polymorphisms for viruses. However, the sequencing depth that guarantees safe interpretation of the observed data is unclear. We demonstrated that 10-fold genome coverage may allow safe genome assembly, and 1000-fold coverage is required to obtain reliable polymorphism estimation.

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