How to use RNA interference | Zendy

Ben Lehner | Zendy

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How to use RNA interference

Author(s) -

Ben Lehner

Publication year - 2004

Publication title -

briefings in functional genomics and proteomics

Language(s) - English

Resource type - Journals

eISSN - 1477-4062

pISSN - 1473-9550

DOI - 10.1093/bfgp/3.1.68

Subject(s) - rna interference , biology , computational biology , small interfering rna , rna , interference (communication) , genetic screen , gene , dna directed rna interference , gene expression , trans acting sirna , genome , genetics , microbiology and biotechnology , computer science , telecommunications , channel (broadcasting) , mutant

RNA interference (RNAi) has been rapidly adopted as a general method for inhibiting gene expression in most laboratory organisms. This paper discusses how libraries of RNAi reagents are being used to perform genome-wide reverse genetic screens in both model organisms and mammalian cells. Guidelines for designing effective small interfering RNAs and appropriate controls for mammalian RNAi experiments will also be discussed.

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