Analysis of protein-protein proximities using the split-ubiquitin system | Zendy

Norbert Lehming | Zendy

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Analysis of protein-protein proximities using the split-ubiquitin system

Author(s) -

Norbert Lehming

Publication year - 2002

Publication title -

briefings in functional genomics and proteomics

Language(s) - English

Resource type - Journals

eISSN - 1477-4062

pISSN - 1473-9550

DOI - 10.1093/bfgp/1.3.230

Subject(s) - biology , ubiquitin , proteolysis , protein fragment complementation assay , complementation , protein–protein interaction , bimolecular fluorescence complementation , microbiology and biotechnology , computational biology , membrane protein , two hybrid screening , genetics , biochemistry , membrane , gene , enzyme , phenotype

The split-ubiquitin system is a fragment complementation assay that is based on a conditional proteolysis design. The system has been used to study protein-protein interactions between membrane proteins and to screen for new interaction partners for transcription factors. This paper outlines the recent progress in the split-ubiquitin technology that has been made possible through the development of new reporter proteins.

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