Improved yeast-screening assay for phytoestrogens | Zendy

Natthida Sophon | Zendy; Choladda Phiwpesh | Zendy; Sieng Darith | Zendy; Wichai Cherdshewasart | Zendy; Chuenchit Boonchird | Zendy

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Improved yeast-screening assay for phytoestrogens

Author(s) -

Natthida Sophon,

Choladda Phiwpesh,

Sieng Darith,

Wichai Cherdshewasart,

Chuenchit Boonchird

Publication year - 2021

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1093/bbb/zbab051

Subject(s) - phytoestrogens , yeast , biology , estrogen , high throughput screening , reporter gene , microbiology and biotechnology , biochemistry , gene , genetics , gene expression

A yeast estrogen screening (YES) assay was improved to increase sensitivity for detection of phytoestrogens. New yeast strains minus one or the other of transporters Pdr5 or Snq2 and harboring yEGFP as a reporter gene were developed. The new strains showed 2-100-fold improvement in sensitivity for detection of standard estrogens and antiestrogens. In addition, the assay time (1 h) using the newly developed strains was shorter than that (4 h) previously reported. Furthermore, the snq2-minus strains were most effective for detection of estrogenic activity while the pdr5-minus strains were most effective for detection of antiestrogenic activity. The efficacy of the new methods was evaluated and confirmed by testing with 23 Thai medicinal plant species. The new strains were also tested for detection of xenoestrogens. The results revealed that the newly developed YES methods were specific and rapid and suitable for simple high-throughput screening or detection of estrogen-like compounds.

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