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How can one increase the biomass of plants, particularly orchids, in an effective way? Photoautotrophic micropropagation is an effective means of increasing the biomass of  Oncidesa  orchids. Using 10,000 µmol mol −1  CO 2  and a photosynthetic photon flux density of 60 µmol m −2  s −1 , the number of leaves and roots and shoot and root fresh and dry weights could be considerably increased, but only when cultured on Kyoto medium rather than Vacin and Went medium. Super-elevated CO 2  enrichment and growth under cold cathode fluorescent lamps can positively affect the efficiency and quality of commercial production of clonal  Oncidesa  plantlets.

Growth of in vitro Oncidesa plantlets cultured under cold cathode fluorescent lamps with super-elevated CO2 enrichment