The trafficking pathway of a wheat storage protein in transgenic rice endosperm
Author(s) -
Mária Oszvald,
László Tamás,
Peter R. Shewry,
Paola Tosi
Publication year - 2014
Publication title -
annals of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.567
H-Index - 176
eISSN - 1095-8290
pISSN - 0305-7364
DOI - 10.1093/aob/mcu008
Subject(s) - endosperm , biology , storage protein , glutenin , genetically modified rice , endoplasmic reticulum , aleurone , microbiology and biotechnology , prolamin , glutelin , vacuole , transgene , organelle , biochemistry , gene , genetically modified crops , cytoplasm , protein subunit
The trafficking of proteins in the endoplasmic reticulum (ER) of plant cells is a topic of considerable interest since this organelle serves as an entry point for proteins destined for other organelles, as well as for the ER itself. In the current work, transgenic rice was used to study the pattern and pathway of deposition of the wheat high molecular weight (HMW) glutenin sub-unit (GS) 1Dx5 within the rice endosperm using specific antibodies to determine whether it is deposited in the same or different protein bodies from the rice storage proteins, and whether it is located in the same or separate phases within these.
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