Microsatellite and flow cytometry analysis to help understand the origin of Dioscorea alata polyploids | Zendy

Alice Némorin | Zendy; Jacques David | Zendy; Erick Malédon | Zendy; Elie Nudol | Zendy; J. Dalon | Zendy; Gemma Arnau | Zendy

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Microsatellite and flow cytometry analysis to help understand the origin of Dioscorea alata polyploids

Author(s) -

Alice Némorin,

Jacques David,

Erick Malédon,

Elie Nudol,

J. Dalon,

Gemma Arnau

Publication year - 2013

Publication title -

annals of botany

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.567

H-Index - 176

eISSN - 1095-8290

pISSN - 0305-7364

DOI - 10.1093/aob/mct145

Subject(s) - biology , ploidy , polyploid , endosperm , gamete , botany , meiosis , parthenogenesis , loss of heterozygosity , apomixis , gametogenesis , sperm , genetics , embryo , allele , cryopreservation , gene

Dioscorea alata is a polyploid species with a ploidy level ranging from diploid (2n = 2x = 40) to tetraploid (2n = 4x = 80). Ploidy increase is correlated with better agronomic performance. The lack of knowledge about the origin of D. alata spontaneous polyploids (triploids and tetraploids) limits the efficiency of polyploid breeding. The objective of the present study was to use flow cytometry and microsatellite markers to understand the origin of D. alata polyploids.

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