Assignment of SNP allelic configuration in polyploids using competitive allele-specific PCR: application to citrus triploid progeny
Author(s) -
José Cuenca,
Pablo Aleza,
Luís Navarro,
Patrick Ollitrault
Publication year - 2013
Publication title -
annals of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.567
H-Index - 176
eISSN - 1095-8290
pISSN - 0305-7364
DOI - 10.1093/aob/mct032
Subject(s) - biology , genotyping , polyploid , genetics , allele , snp genotyping , ploidy , snp array , snp , genotype , single nucleotide polymorphism , gene
Polyploidy is a major component of eukaryote evolution. Estimation of allele copy numbers for molecular markers has long been considered a challenge for polyploid species, while this process is essential for most genetic research. With the increasing availability and whole-genome coverage of single nucleotide polymorphism (SNP) markers, it is essential to implement a versatile SNP genotyping method to assign allelic configuration efficiently in polyploids.
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