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Gene dosage effect of WEE1 on growth and morphogenesis from arabidopsis hypocotyl explants
Author(s) -
Natasha D. Spadafora,
Lara Perrotta,
Jeroen Nieuwland,
Diego Albani,
Maria Beatrice Bitonti,
Robert J. Herbert,
John H. Doonan,
Angela Marchbank,
Ilario Siciliano,
Anne Lentz Grønlund,
Dennis Francis,
Hilary J. Rogers
Publication year - 2012
Publication title -
annals of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.567
H-Index - 176
eISSN - 1095-8290
pISSN - 0305-7364
DOI - 10.1093/aob/mcs223
Subject(s) - wee1 , biology , meristem , hypocotyl , botany , cell cycle , morphogenesis , microbiology and biotechnology , cell , genetics , gene , cyclin dependent kinase 1 , shoot
How plant cell-cycle genes interface with development is unclear. Preliminary evidence from our laboratory suggested that over-expression of the cell cycle checkpoint gene, WEE1, repressed growth and development. Here the hypothesis is tested that the level of WEE1 has a dosage effect on growth and development in Arabidospis thaliana. To do this, a comparison was made of the development of gain- and loss-of-function WEE1 arabidopsis lines both in vivo and in vitro.

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