Two New Nuclear Isolation Buffers for Plant DNA Flow Cytometry: A Test with 37 Species | Zendy

João Loureiro | Zendy; Eleazar Rodriguez | Zendy; Jaroslav Doležel | Zendy; Conceição Santos | Zendy

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Two New Nuclear Isolation Buffers for Plant DNA Flow Cytometry: A Test with 37 Species

Author(s) -

João Loureiro,

Eleazar Rodriguez,

Jaroslav Doležel,

Conceição Santos

Publication year - 2007

Publication title -

annals of botany

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.567

H-Index - 176

eISSN - 1095-8290

pISSN - 0305-7364

DOI - 10.1093/aob/mcm152

Subject(s) - biology , nuclear dna , propidium iodide , flow cytometry , herbaceous plant , dna , dna extraction , botany , microbiology and biotechnology , biochemistry , mitochondrial dna , polymerase chain reaction , apoptosis , programmed cell death , gene

After the initial boom in the application of flow cytometry in plant sciences in the late 1980s and early 1990s, which was accompanied by development of many nuclear isolation buffers, only a few efforts were made to develop new buffer formulas. In this work, recent data on the performance of nuclear isolation buffers are utilized in order to develop new buffers, general purpose buffer (GPB) and woody plant buffer (WPB), for plant DNA flow cytometry.

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